Submited on: 24 Jan 2013 05:49:57 PM GMT
Published on: 25 Jan 2013 08:56:34 AM GMT

  • What are the main claims of the paper and how important are they?

    This paper provides a novel method for conducting ELISA in order to determine the relative stoichiometry and affinity of von Willebrand Factor (vWF) binding to coagulation factor VIII (FVIII) with the intention of creating a simple home test of vWF activity in patients with von Willebrand Disease type 2N.

  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    While other papers (cited by the authors) have shown novel methods for testing the relationships between vWF and FVIII, the authors provide a simple method, which can be used for testing in research laboratories. 

  • Are the claims properly placed in the context of the previous literature?

    The authors provide adequate literature to support their claim that their method is simpler/more economically sound.

  • Do the results support the claims? If not, what other evidence is required?

    The authors do not provide comparisons with other ELISA tests for vWF/FVIII interactions and it is therefore not possible to determine the validity of their claim that their method is easier. Nor does it provide a compairson of the accuracy of their method with other methods.

  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    The mothodology described in the paper is thoroughly explained. However, there was no assessment of the removal of FVIII from the vWF and it is therefore difficult to assess the accuracy of the method based on the provided information.

  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    A potential test for the presence of FVIII after washing Wilate(r) might have been to conduct another ELISA on the pretreated vWF/FVIII after FVIII removal to look for the presence of FVIII. Studies comparing existing ELISA tests with the one presented in this paper, may help support the validity of the method, as well as the author's claim that their method is 'easier' to conduct.

  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    This paper does not provide groundbreaking information in the field of haematology or von Willebrand Disease. However, it does provide a simple and potentially useful method that can be replicated in academic research settings when conducting studies with vWF.

  • Other Comments:


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  • Have you previously published on this or a similar topic?:
  • References:
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  • How to cite:  Nardo D .Review of Relative Stoichiometry and Affinity for Describing Von Willebrand Factor Binding of Coagulation Factor VIII[Review of the article 'Relative Stoichiometry and Affinity for Describing Von Willebrand Factor Binding of Coagulation Factor VIII ' by Pachmann U].WebmedCentral 2017;8(11):WMCRW003388
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