Submited on: 13 Sep 2012 07:14:58 AM GMT
Published on: 13 Sep 2012 05:45:39 PM GMT
 
Is zinc deficiency an independent risk factor in causation of
Posted by Anonymous Reviewer on 04 Oct 2012 04:02:31 PM GMT

  • What are the main claims of the paper and how important are they?

    Less attention to micronutrients especially zinc, which leads to oxidative stress induced IHD. zinc suppliments required for IHD.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    Yes


  • Are the claims properly placed in the context of the previous literature?

    Yes


  • Do the results support the claims? If not, what other evidence is required?

    Yes


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    For a randomized trial, some deviations may be there, but the authors have adequately explained such occurances.


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Very much valid and more than enough details in its support.


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    Its sufficient in itself.


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    Yes, because it shows that zinc defficiency as the earlier marker of oxidative stress which may lead to IHD.


  • Other Comments:

    Its benefits for the future prospects towards the prevention of IHD.

  • Competing interests:
    No
  • Invited by the author to review this article? :
    Yes
  • Have you previously published on this or a similar topic?:
    No
  • References:
  • Experience and credentials in the specific area of science:

    9 YEARS

  • How to cite:  Anonymous.Is zinc deficiency an independent risk factor in causation of [Review of the article 'Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease ' by Ramessur K].WebmedCentral 2012;3(10):WMCRW002279
1 2 3 4 5 6 7 8 9
Report abuse
 

  • What are the main claims of the paper and how important are they?

    Study answered that low zinc level observed in IHD patients correlate with oxidative stress markers. Several other studies have indicated that low zinc level may predispose to endothelial dysfunction. However authors in the present study asserted that zinc supplementation would prevent oxidative stress, this can not be supported by data provided. 


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    The claims are an addition to the existing controversies regarding the status of zinc supplementation in relation to development of heart disease.

    1. Wael K. Al-Delaimy, Eric B. Rimm, Walter C. Willett, Meir J. Stampfer,and Frank B. Hu (2004). Magnesium Intake and Risk of Coronary Heart Disease among Men J Am Coll Nutr23:63-70
    2. Cara L. Eckhardt (2006). Micronutrient Malnutrition, Obesity, and Chronic Disease in Countries Undergoing the Nutrition Transition: Potential Links and Program/Policy Implications 2033.

    http://www.ifpri.org/sites/default/files/pubs/divs/fcnd/dp/papers/fcndp213.pdf


  • Are the claims properly placed in the context of the previous literature?

    Partially. Some speculations made can not be substantiated.


  • Do the results support the claims? If not, what other evidence is required?

    Not absolutely to the extent of the speculations made by authors.


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    NA


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Detailed


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    Determination of endothelial nitric oxide (NO) level. NO production is needed for maintanance of the vascular bed and studies have shown that impaired production of leads to endothelial thickening prerequisite to atherosclerosis. Oxidative stress has been identified as a factor that could repress the expression of endothelial nitric oxide synthase, the enzyme responsible for NO synthesis.


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    Science of study is sound.


  • Other Comments:

    NA

  • Competing interests:
    None
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    Study falls within biochemistry specialty

  • How to cite:  Anonymous.Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease [Review of the article 'Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease ' by Ramessur K].WebmedCentral 2012;3(9):WMCRW002251
1 2 3 4 5 6 7 8 9
Report abuse
 

  • What are the main claims of the paper and how important are they?

    The paper claims that zinc deficiency can induce oxidative stress and can progress to atherosclerosis. Evaluating the functional role of zinc deficiency in the induction of oxidative stress in tissues leading to heart disease has clinical importance.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    The claims are not novel.  The research conducted in human subjects is supportive to previously investigated researches elsewhere. Nutritional zinc deficiency has been investigated by other investigators at various aspects searching for various parameters including serum levels of zinc, role of zinc in in the induction of oxidative stress, as cofactor for superoxide dismutase and other enzymes, and the lipid profiles. However, this paper combined few parameters to be tested during zinc deficiency and data obtained supports earlier investigations.


  • Are the claims properly placed in the context of the previous literature?

    The claims are not new. Previous literature and studies investigated the parameters studied in this paper either independently or collectively.


  • Do the results support the claims? If not, what other evidence is required?

    The results obtained in this paper support the claims.


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    Both the significant and insignificant results were explained with appropriate statistical analysis. Author explained the deviations.


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Methodologies used are valid.

    Not enough details about the methodology were described, except the relevant reference to them. The group of patients as designed does not always match in text and in table and needs to be clarified in details. Such as, illustration 2 does not describe about Group A, Group B and Group C in the text or in the table. Illustration 3 describes Group-I, Group-II and Group-III. These are not described in the research design section and needs to be addressed. There are few mistakes in writing and in abbreviation, such as “high density lipoprotein-cholesterol” (HDLc) etc. in paragraph 2 of the research design section.


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    This study could have been improved by studying the effect of supplementation of dietary zinc over time (to be standardized) and observe if the patients improve their clinical and biochemical conditions.


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    This paper is not outstanding in its discipline. It is more supportive or confirmative of previously conducted research.


  • Other Comments:

    NA

  • Competing interests:
    No
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
  • Experience and credentials in the specific area of science:

    Nutritional biochemistry

  • How to cite:  Sarkar P K.Nutritional Zinc Impairment in Ischemic Heart Disease.[Review of the article 'Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease ' by Ramessur K].WebmedCentral 2012;3(9):WMCRW002243
1 2 3 4 5 6 7 8 9
Report abuse
 

  • What are the main claims of the paper and how important are they?

    The paper claims that serum zinc, SOD and MDA levels can be used as independent markers for early detection of oxidative stress and IHD.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    These claims are interesting and deserve further study but they are not novel. Here are a few papers that have studied similar claims:

    1. Study of oxidative stress and trace element levels in patients with alcoholic and non-alcoholiccoronary artery disease.
    Dey Sarkar P et al
    Indian J Physiol Pharmacol. 2007
    2. Zinc deficiency, ethanol, and myocardial ischemia affect lipoperoxidation in rats.
    Coudray C et al
    Biol Trace Elem Res. 1991
    3. Malondialdehyde-modified low-density lipoprotein is a useful marker to identify patients with vulnerable plaque.
    Tajika K, et al
    Circ J. 2012
    4. The combined transduction of copper, zinc-superoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury.
    Huang GQ, et al
    J Transl Med. 2011


  • Are the claims properly placed in the context of the previous literature?

    Absolutely the claims are in context with the previous literature and experiments bolster previous studies.


  • Do the results support the claims? If not, what other evidence is required?

    The claims are more or less supported by the evidence however the paper could have addressed a few concerns:

    1. IHD patients have low zinc levels and SOD activity but they also have high LDL, cholesterol, triglycerides and low HDL. So zinc levels cannot be considered as an independent marker for IHD.

    2. Vitamin E prevents peroxilation of fatty acids and lowers MDA. Vit E levels in these patients would have been a nice addition.


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    There are no deviations from the trial.


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    The methodology is valid and the paper offers enough details of its experiments to reproduce the results.


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    Yes the paper would have been outstanding if certain extra work was done:

    1. Serum levels of Nitirc oxide Synthase (NOS), Xanthine Oxidase (XO), and glutathione S-transferase (GST)

    2. Serum levels of TNF-alph, interleukins and Vit E.

    3. peroxylipid, thioredoxin, 8- hydroxy deoxyguanosine and ferritin levels in serum point to the cause of the oxidative stress.


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    The paper is well written but not oustanding in its field.


  • Other Comments:

    The paper is very well written. the experimental procedures are clear and statistics well explained.

  • Competing interests:
    None
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
  • Experience and credentials in the specific area of science:

    I am a Post-doc in Immunology specializing in oxidative stress and cell death.

  • How to cite:  Dotiwala F J.A case control study for zinc as a risk factor in IHD[Review of the article 'Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease ' by Ramessur K].WebmedCentral 2012;3(9):WMCRW002238
1 2 3 4 5 6 7 8 9
Report abuse
 

  • What are the main claims of the paper and how important are they?

    The paper studied Zinc Deficiency in Ischemic Heart Disease. It is interesting.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    Yes. Some claims are novel.


  • Are the claims properly placed in the context of the previous literature?

    Yes.


  • Do the results support the claims? If not, what other evidence is required?

    Yes. The results support the claims


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    There are not impotant deviations.


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Correlation analysis in  statistics should be used in the markes which author have detected. TC, TG, HDL-C,VLDL-c, LDL-C,  Malondialdehydelevel, Superoxide dismutase enzyme activity should do Correlation analysis in  statistics.


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    Correlation analysis in  statistics should be used in the markes which author have detected.


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    Yes. This paper is some outstanding in its field.


  • Other Comments:

    English should be impoved.

  • Competing interests:
    None
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:

    No

  • Experience and credentials in the specific area of science:

    No

  • How to cite:  Liu C .Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease [Review of the article 'Is Zinc Deficiency an Independent Risk Factor in the Causation of Ischemic Heart Disease? A case Control Prospective Study to Estimate Serum Zinc Levels in Patients of Ischemic Heart Disease ' by Ramessur K].WebmedCentral 2012;3(9):WMCRW002233
1 2 3 4 5 6 7 8 9
Report abuse