Submited on: 28 May 2012 12:40:53 PM GMT
Published on: 29 May 2012 05:13:40 PM GMT
 
Vitamin E Prevents Lipogenesis Dysregulation in the Liver Cells at Old Age
Posted by Anonymous Reviewer on 27 Jun 2012 05:44:27 AM GMT

  • What are the main claims of the paper and how important are they?

    1. Age-dependent lipogenesis dysregulation depends on redox state changes in liver

    2. Vitamin E and N-acetylcistein (NAC) increased liver and hepatocytes sensitivity to T4 action in old age

     

    Both findings are quite important.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    1. Not really
    2. Links of other papers with findings of age-related changes in liver lipogenesis
    3. But the findings of Vit E and NAC action is a novel finding and is quite important  
         (i)http://dx.doi.org/10.1016/0020-711X(83)90173-8,
         (ii)Diabete Metab. 1990 Jul-Aug;16(4):290-5
         (iii)Am J Physiol Endocrinol Metab (August 5, 2008).                       doi:10.1152/ajpendo.90438.2008- HEPATIC REGULATION OF FATTY ACID SYNTHASE BY INSULIN AND T3: EVIDENCE FOR T3 GENOMIC AND NON-GENOMIC ACTIONS.


  • Are the claims properly placed in the context of the previous literature?

    Yes


  • Do the results support the claims? If not, what other evidence is required?

    Yes. But evidence regarding claim about"age-dependent lipogenesis dysregulation depends on redox state changes in liver" and their sensitivity depends on Vit E& NAC states is not complete,since other factors such as developmental nutritional states/factors [role of choline in NASH,phosphatidylcholine synthesis,PUFA etc] do also contribute to age-dependent dysregulation  


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    NA


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Yes


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    It would be interesting to see the developmental effects of the same & their importance in age-related lipogenesis dysregulation


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    The finding is interesting.


  • Other Comments:

    Few typographical errors  noted.

  • Competing interests:
    No
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    Working with Dr Steven Zeisel at UNC Chapel Hill,I'm aware of the role of choline in development of NASH.

  • How to cite:  Anonymous.Vitamin E Prevents Lipogenesis Dysregulation in the Liver Cells at Old Age [Review of the article 'Vitamin E Prevents Lipogenesis Dysregulation in the Liver Cells at Old Age ' by Garkavenko V].WebmedCentral 2012;3(6):WMCRW001993
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Methodology and Content
Posted by Dr. James J Bulot on 26 Jun 2012 03:35:05 PM GMT

  • What are the main claims of the paper and how important are they?

    Not able to rate


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    Not able to rate


  • Are the claims properly placed in the context of the previous literature?

    There are references in the discussion/conclusion to "Different vitamin E constituents lipid-lowering properties have been demonstrated in cell lines (Parker et al., 1993), animal models (Qureshi et al., 2005) and humans (Qureshi et al., 1991).  " it would seem useful to use these findings in the introduction to set the framework for further investigation in Vit E  and it's relation to lipid-lowering properties as well as for how the methodology might differ from previous studies. 


  • Do the results support the claims? If not, what other evidence is required?

    Not able to rate


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    Statistical analysis included a one-way analysis of variance (ANOVA) followed by post hoc Fisher’s protected least significant difference.  Does the theoretical framework support this type of analysis (Anova)?  I would expand on the rationale behind using the anova as the analytical method


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Not able to rate


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    Not able to rate


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    Not able to rate


  • Other Comments:

    Check spelling: The significant age-dependent decrease of ACC and FAS mRNA expression and their induction by reffeeding has been determined in the liver of fasted rats (Fukuda and Iritani, 1992).  Unsure if this "?" is intentional: 24-month-old rats and effects of vitamin E (?-tocopherol acetate) 



  • Competing interests:
    No
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    Aging, statistics, research methods

  • How to cite:  Bulot J J.Methodology and Content[Review of the article 'Vitamin E Prevents Lipogenesis Dysregulation in the Liver Cells at Old Age ' by Garkavenko V].WebmedCentral 2012;3(6):WMCRW001985
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Vitamin E Prevents Lipogenesis
Posted by Prof. Heidrun Karlic on 26 Jun 2012 10:04:51 AM GMT

  • What are the main claims of the paper and how important are they?

    NA


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    NA


  • Are the claims properly placed in the context of the previous literature?

    NA


  • Do the results support the claims? If not, what other evidence is required?

    NA


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    NA


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    NA


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    NA


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    NA


  • Other Comments:

    The correct gene name for fatty acid synthase is FASN. FAS (as written in this paper) refers to an apoptosis mediator also known as CD95

     

  • Competing interests:
    No
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    Yes
  • References:

    Karlic H, Lohninger S, Koeck T, Lohninger A. Dietary l-carnitine stimulates carnitine acyltransferases in the liver of aged rats. J. Histochem. Cytochem. 2002;50:205-212.

  • Experience and credentials in the specific area of science:

    Nutritional, physiological and molecular aspects which may promote the susceptibility for malignancies in advanced age

  • How to cite:  Karlic H .Vitamin E Prevents Lipogenesis[Review of the article 'Vitamin E Prevents Lipogenesis Dysregulation in the Liver Cells at Old Age ' by Garkavenko V].WebmedCentral 2012;3(6):WMCRW001984
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1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? Yes
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? No
7 Can you suggest any reductions in the paper, or deletions of parts? No
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? Yes
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

    Very important paper for its contribution!

  • Competing interests:
    no
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    I am linked with anti ageing medicine.

  • How to cite:  Bedin V .Vitamin E prevents lipogenesis dysregulation in the liver cells at old age[Review of the article 'Vitamin E Prevents Lipogenesis Dysregulation in the Liver Cells at Old Age ' by Garkavenko V].WebmedCentral 2012;3(5):WMCRW001839
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