The publication, “An Overview of the Basics of Generate Transgenic Hen 'Bioreactors'”, describing the artificial induction ( genetic engineering ) of human monoclonal antibody in eggs of transgenic hens, may open again the intriguing question how to explain former ( now almost historical) data of experiments performed on C57BL/10J unfertilized female mice, which exclusively produced a “natural” anti-histo-blood group-A antibody, either in conjunction with /or as response to its ovarian auto-reactive epitope  http://www.nature.com/nature/journal/v269/n5625/abs/269255a0.html.

Any interpretations of this old, but perhaps physiologically relevant observation remained up to now incomplete. In particular, the protein- and/or immunoglobulin classes, the cellular and topographical site of production of the murine “natural” anti-A antibody are still an open question. In fact, early ovariectomy, performed before the onset of puberty, always caused its immediate disappearance from the sera. This phenomenon, i.e. the rapid or even immediate antibody disappearance could hardly be explained by simply a removal of an antigenic stimulus combined with changing the hormonal situation. So the well known occurrence of “natural” antibody or “antibody-like” proteins, for instance, in fish eggs,  may raise a vague hypothesis of an ovarian site of spontaneous antibody production here even in a mammalian species, close to the auto-reactive epitope or in a molecular conjunction with it.

Genetic engineering may finally elucidate the mechanism, the cellular and topographical site of production of this particular murine “natural” anti-A antibody, and on this basis perhaps improve the knowledge about germline encoded “natural” antibodies, and should the situation arise, in bioreactors perhaps prepare their manufacturing.

None, but earlier own work touches this area and opens new questions

Safety issues such as harmful outcomes, preventative measures, and long-term effects on both poultry and humans may add to the value and quality of a very interesting review paper. Previous research in this arena raises biosafety issues. The risks can be mitigated by the nature of the vector system (and its safety features) or exacerbated by the nature of the transgene insert encoded by the vector. This being virtually impossible to predict must be a concern due to outcome and resultant effects obeserved when cells mature.

None

This is in response to the invitation for review from Lead faculty and one of the author (the section below did not show it corectly).

It is a review, which describes "transgenic hen". Although there are some rough points, it is a good one. The figures are colorful and nice.

have more than 30 papers in the field of transformation and transfenics in model systems (aging, stress resistance, and others). no experience in "hen".