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Dr. Ajay Singh

European School of Molecular Medicine (SEMM)
via adamello 16, Milan

Brief Biography:


Academic positions:

01 Jan 2008-31 Dec 2011 : European School of Molecular Medicine (SEMM), Milan Italy PhD, Medical Nanotechnology
Supervisor: Prof. Paolo Milani Director, Filarate Foundation, University of Milan, Italy.
Co-supervisor: Prof. Cristina Lenardi Head, MicroNanofabrication Unit, Filarate Foundation, University of Milan, Italy
Research project: Designing a new recipe for cell-protein interactions.
Cell-surface interactions are fundamental to in vivo implant biology, biosensor, cell microarray and performing cell biology to chip format. Controlling material surface features at nanoscale length to optimize cell response is instrumental to success of biomedical device/implant. Using pulsed microplasma cluster source (PMCS) equipped with supersonic cluster beam (SCBD) which is a home built facility at our lab; we developed novel protocols for micro-nanostructuring of solid state materials (TiOx/ZrOx/CNx) in chip format. The novelty of the technique lies in its facility of decoupling nanoscale topography with surface chemistry and vice versa to study the influence of surface physico-chemical properties on the performance of the materials in contact with complex biological environment. The most significant step of my PhD dissertation is the development of high throughput approach for the nanostructured thin film synthesis and characterization. We developed recipes for novel solid state material library design with gradient in nanoscale morphology roughness and wettability, based on beam engineering of PMCS/SCBD apparatus. Further, using multiplex fluorescence microscopy, probed the “hit spots” for optimum material surface-protein-cell interactions using prokaryotic and eukaryotic (mammalian and bacterial) cells with high content image analysis software (ImageJ, IMARIS 7.0, Bitplane's core system). On single material library with more than 1000 gradient spot array, it is possible to predict the optimum material surface properties to promote mammalian cell-interaction and/or inhibit bacterial colonization and vice versa. Technique routinely used so far polymeric material design, proposes new avenues for solid state materials development and discovery.
01 Jun 2003-31 May 2005 : University of Pune, India MSc, Biotechnology
Supervisors: Dr. Murali Sastry and Dr. BLV Prasad National Chemical laboratory, Pune, India
Research Project: Synthesis of gold, silver and their alloy Nanoparticles using Bovine Serum Albumin as foaming and stabilizing agent.
September 2000-April 2003 : Avadh Dental College, Lucknow, Uttar Pradesh, India
Bachelor in Oral Medicine (Human Anatomy, pathology, Pharmacology, Human physiology, Dental Materials, Basic Internal Medicine)
May 1995-April 1998 : Kamla Nehru Institution, Sultanpur, Uttar Pradesh, India
BSc, Life Sciences (Zoology, Botany and Chemistry)
Aug 2006-Dec 2007 : Santana Hospital, Ferrara School of Medicine, Ferrara, Italy
Position held: Research Assistant Principle Investigator: Prof. Paolo  Zamboni Screening of gene variants in Multiple Sclerosis by electronic microarray using nanogen molecular biology nanochip and Synergistic role of iron overload and FXIII gene variants in Venous leg Ulcer (VLU).
Sep 2005-Jun 2006 : Department of Biotechnology, University of Pune, India
Position held: Junior Research Fellow Principle Investigator: Prof. W.N. Gade
Project: Molecular mechanism involved microbial based synthesis of nanoparticles using bacteria and fungi.


Research interests:

Laboratory based
Material Fabrication: Colloidal synthesis and characterization of metal nanoparticles via chemical and green (microbial/plant) routes
Operation and handling of pulsed microplasma cluster source (PMCS) for supersonic cluster beam deposition (SCBD) of nanostructured thin film of transition metal oxides (titanium, zirconium, carbon). Atomic force microscopy (AFM) for nanoscale morphology characterizations, Optical prifilometry analysis, and water contact angle (WCA) measurement.
Developed high throughput (HTP) approaches for synthesis and characterization of material gradient library of TiOx/ZrOx/CNx and nanostructured surface-protein-surface interactions on single chip. High content optical profilometric analysis using Apex 2DTM  software for nanoscale step thickness, rms roughness and surface skewness analysis.
Biological Assays: Nanoparticle-cell interactions assays for cytotoxicity evaluation (cell culture: PC12, MG63, SHSY5Y, HeLa, MDCK, U2OS cell lines.
Molecular biology and Microscopy techniques: MTT assay, protein extraction purification, SDS-PAGE for protein identifications bright filed/confocal scanning laser microscopy (CLSM).
Designed and developed micro-nanostructured platform to control cell-surface interaction on chip for cell assays and implants surface optimization. During training, pursued different polymer spin coating, lithography, routine mammalian cell culture protocol and viability assays (Annxin V/MTT), immunostaning, CLSM. Multi content fluorescent microscopy with full automations.
Multiplex Image analysis software: ImageJ, cell profiler, Imeris v.7, BITPLANE core software. Microsoft Office, Graphpad Prism, Coreldraw, Endnote and Origin.
1.November 27- December 2, 2011, MRS Fall Meeting Symposium at Boston. Scheduled Oral presentation “Bacterial Adhesion and Biofilm Formation on Nanostructured Titanium Oxide Surfaces”.
2.Materials Research Society, Spring symposium. Poster presentation. Rapid Prototyping of nano- and micro-patterned substrates for the control of PC12 cells neuritogenesis by topographic and chemical cues (April, 2010), San Francisco, USA.
3.Trans-alpine Conference in Nanoscience and Nantechologies.  Poster Presentation. Neurons sense and respond to extensive neurite branching on patterned BSA islands generated by nanosphere lithography. (June, 2010), Como, Italy.
4.International Conference of Nanobiosciences. Poster Presentation. Biological Synthesis of CuO Nanoparticles using bacteria E.coli (August, 2006), Pune, India.


Any other information:

1.Ajay V Singh, Erica Montani, Varun Vyas, Tushar S Salve, Daniele Cortelli, Sara Barozzi, Dario Parazzoli Paolo Milani, *Cristina Lenardi. A high throughput methodology to investigate nanostructure surface-protein-cell interactions on inorganic material libraries [Manuscript under preparation]
2.Ajay Vikram Singh, Varun Vyas, Rajendra Patil, PE Scopelliti, Vimal Sharma, Gero Bongiorno, Alessandro Podesta, Cristina Lenardi, *WN Gade,*Paolo Milani. Quantitative characterization of the influence of nanoscale morphology on bacterial adhesion and biofilm formation on nanostructured titanium oxide surfaces. PLoS ONE 2011; 6(9): e25029.
3.Ajay V Singh, Lasma Gailite, Varun Vyas, Cristina Lenardi, Stefania Forti, Michela Matteoli, Paolo Milani. Rapid prototyping of nano- and micro-patterned substrates for the control of cell neuritogenesis by topographic and chemical cues. Journal of Material Science and Engineering: C 2011; 32:892-899.
4.Ajay V Singh, R Patil, Atul Anand, Paolo Milani and WN Gade. Biological Synthesis of Copper Oxide Nano Particles Using Escherichia coli. Current Nanoscience 2010; 6 (4): 365-369.
5.AV Singh, C Lenardi, L Gailite, A Gianfelice and P Milani. A simple lift-off-based patterning method for micro- and nanostructuring of functional substrates for cell culture. J. Micromech. Microeng. 19 (2009) 115028-35.
6.AV Singh, R Patil, WN Gade, BLV Prasad. Synthesis of Ag-Pt Alloy Nanoparticles in Aqueous Bovine Serum Albumin Foam and their Cytocompatibility against Human Gingival Fibroblasts. Colloids and Surfaces B: Biointerfaces 69 (2009) 239–245.
7.Ajay V Singh, Bapurao M Bandgar, Manasi Kasture, BLV Prasad, Murali Sastry. Synthesis of gold, silver and their alloy Nanoparticles using bovine serum albumin as foaming and stabilizing agent. J Mater Chem, 2005, 15, 5115–5121.
Invited Reviews/Commentaries:
1.Ajay V Singh, Anisur Rahman, NVG Sudhir Kumar, Aditi AS, M Galluzzi, S Bovio, S Barozzi, E Montani, D Parazzoli. Bio-inspired approaches to design smart fabrics. Journal of Materials and Design (2011), doi:10.1016/j.matdes.2011.01.061 [In Press].
2.Ajay V Singh, Snigdha Maheshwari, Dario Giovanni, Venkatesh G Naikmasur, Arpita Rai, Aditi VP, W.N.Gade, Varun Vyas, Donato Gemmati,Giulia Zeri, Elisa Orioli. Nanoengineering approaches to design advanced dental materials for clinical applications. Journal of Bionanosciences 2010; 4: 1-13.
3.Ajay V Singh, Aditi AS, W.N. Gade, Tanushree Vats, Cristina Lenardi and Paolo Milani. Nanomaterials: New Generation Therapeutics in Wound Healing and Tissue Repair. Current Nanoscience, 2010, 6, (6):577-586.
4.Ajay V Singh, Subhashree L, Paolo Milani, Donato Gemmati, Paolo Zamboni. Interplay of iron metallobiology, metalloproteinases, FXIII and role of their gene variants in venous leg ulcer. The International Journal of Lower Extremity Wounds 2010; 9 (4):166-179.
5.Ajay Vikram Singh and Paolo Zamboni. Anomalous venous blood flow and iron deposition in multiple sclerosis. Journal of Cerebral Blood Flow & Metabolism 2009; 29: 1867-1878.
6.Uttara Bayani, Ajay V Singh, P. Zamboni, R.T. Mahajan. Neurodegenerative Diseases and Oxidative Stress: Review of Upstream and Downstream Therapeutic Options. Current Neuropharmacology 2009;7(1):65-74.
7.S Lanzara, L Catozzi, M De Palma, F Federici, A Palazzo, G. Tacconi, S Gianesini, AV Singh, P Zamboni. Mechanism of Disease: the inflammatory chain in chronic venous disorder and genetic screening for prevention of venous leg ulcers. ACTA PHLEBOLOGICA 2007; 8 (94): 1-6.
Book chapters:
1.Ajay V Singh, Rajendra Patil, Cristina Lenardi, Paolo Milani and WN Gade*. Nanobiomaterial Applications in Tissue Repair and Ulcer Management: A New Role for Nanomedicine. In:  Biocompatible Nanomaterials: Synthesis, Characterization and Applications: edited by S. Ashok Kumar, Soundappan Thiagarajan and Sea-Fue Wang (National Taipei University of Technology, Taipei, Taiwan); Nova Science Publishers, New York.
2.Ajay V Singh and Paolo Zamboni. Genetic delineation of venous leg ulcer and its clinical implications, edited by Prof. Roger Greenhalgh, published by BIBA, London, UK.
3.Ajay V Singh, Paolo Zamboni and WN Gade. Nanotechnology and Nanomedicine: A novel tool in diagnosis and therapy in abnormal folate pathway related malignancies, edited by  Doanto Gemmati, published by research sign post Kerala, India.
4.Corresponding Author
1.Prof.Paolo Milani
Email: paolo.milani@mi.infn.it
Phone: +39 02 5031.17219
Fax: +39/0250/317482
Fondazione Filarete, viale Ortles 22/4, I-20139 Milano, Italy.
2.Prof. Cristina Lenardi
Email: cristina.lenardi@mi.infn.it
Phone: +39 02 5031.17219
Fax: +39/0250/317482
CIMAINA and Dipartimento di Scienze Molecolari Applicate ai Biosistemi, Universita’ di Milano, via Trentacoste 2, I-20134 Milano, Italy
3.Prof. WN Gade
Email: wngade@unipune.ac.in
Ph: +91 20 25694952  
Fax: +91/20/25691821
Department of Biotechnolyg, University of Pune, Ganesh Khind, Pune, 411007, Maharashtra, India    
4.Dr  Nikolaj Gadegaard
Email: N.Gadegaard@elec.gla.ac.uk
Phone: +44 (0)141-330 5243
Fax:  +44 (0)141-330 4907
Department of Electronic & Electrical Engineering, Jameswat Building, University of Glasgow, G12 8LT

I enjoy reading science philosophy, digital photography, playing outdoor games, cooking and dance as a hobby.


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