I appreciate the references concerning our article introduced by the reviewer, Dr. Tomonaga. Especially, Vriens et al's article was very interested for me. They classified four distinct subsets of heat-sensitive neurons. The largest group of them is heat-sensitive neurons that respond to both neuroactive steroid pregnenolone sulfate (PS) and capsaicin, suggesting coexpression of TRPV1 and TRPM3. Second and third are heat-sensitive neurons that respond to capsaicin but not PS (TRPV1-expressing), or to PS but not to capsaicin (TRPM3).  Fourth, a fraction of heat-activated neurons unresponsive to both PS and capsaicin, indicating the existence of a TRPM3- and TRPV1-independent heat-sensing mechanism. From this, it seems that the remaining neurons after capsaicin treatmen are their fourth type heat-sensitive neurons, accordingly the animals treated with capsaicin at neonate can sense noxious heat normally.