Home » Reviews
Amplification, Cloning And Characterization Of Rna Helicase Gene From Aedes Aegypti
By Mr. Ahmad Ibrahim , Dr. Wajidi M. F. F. , Mrs. Sawsan S. Abdul Hameed , Dr. Amin Malik Shah Abdul Majid
Submited on: 30 Oct 2010 10:11:51 AM GMT
Published on: 30 Oct 2010 10:27:56 PM GMT
 
Amplification, Cloning And Characterization Of Rna Helicase Gene From Aedes Aegypti
Posted by Dr. Siddharth K Tripathi on 01 May 2013 02:10:04 AM GMT

  • What are the main claims of the paper and how important are they?

    Authors claimed that they The present study reported the amplification, cloning and determination of nucleotide sequences of the gene that encodes for DEAD box ATP-dependent RNA helicase, from Ae. aegypti.


  • Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.

    No. Genome sequence of Aedes aegypti, a major arbovirus vector. Nene V, et al. Science, 2007 Jun 22. PMID 17510324.


  • Are the claims properly placed in the context of the previous literature?

    Yes


  • Do the results support the claims? If not, what other evidence is required?

    Yes. 


  • If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?

    No


  • Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?

    Yes


  • Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?

    NA


  • Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?

    No. The paper is not outstanding as it is with preliminary and common data.


  • Other Comments:

    I would like to advise the author to be cautious,

    1. Authors didn’t characterize cloned DEAD box RNA helicase as they mention in paper title.
    2. Authors claimed that they used genomic DNA for the cloning of DEAD box RNA helicase, instead of genomic DNA they could use cDNA.

  • Competing interests:
    None
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    Studied RNA helicases

  • How to cite:  Tripathi S K.Amplification, Cloning And Characterization Of Rna Helicase Gene From Aedes Aegypti[Review of the article 'Amplification, Cloning And Characterization Of Rna Helicase Gene From Aedes Aegypti ' by Shah Abdul Majid A].WebmedCentral 2013;4(5):WMCRW002710
1 2 3 4 5 6 7 8 9
Report abuse
 
 
 
We use cookies on this website to measure and improve performance. By continuing to browse the site, you are agreeing to our use of cookies.