Submited on: 13 Sep 2010 11:20:23 PM GMT
Published on: 14 Sep 2010 12:56:31 AM GMT
 

1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? Yes
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? Yes
7 Can you suggest any reductions in the paper, or deletions of parts? No
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? Yes
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

     

    Identifying the role cancer stem cells (CSC) is important in understanding the resistance to standard therapy of surgery, radiation and TMZ. Standard glioma cell lines, U87 has been used for a long time and understanding the role of CSC in these lines is important. The above procedure to isolate CSC will be critical in understanding GBM biology

  • Competing interests:
    no
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    Yes
  • References:
    None
  • Experience and credentials in the specific area of science:

    Working in glioma stem cells and  potential  therapeutic applications

  • How to cite:  Sathyan P .Isolation of Cancer stem cells from GBM cell lines[Review of the article 'An efficient approach to Enrich Glioma Stem Cells from Glioma Cell Lines in Culture ' by Kamnasaran D].WebmedCentral 2010;2(3):WMCRW00531
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1 Is the subject of the article within the scope of the subject category? Yes
2 Are the interpretations / conclusions sound and justified by the data? Yes
3 Is this a new and original contribution? Yes
4 Does this paper exemplify an awareness of other research on the topic? Yes
5 Are structure and length satisfactory? Yes
6 Can you suggest brief additions or amendments or an introductory statement that will increase the value of this paper for an international audience? No
7 Can you suggest any reductions in the paper, or deletions of parts? No
8 Is the quality of the diction satisfactory? Yes
9 Are the illustrations and tables necessary and acceptable? Yes
10 Are the references adequate and are they all necessary? Yes
11 Are the keywords and abstract or summary informative? Yes
  • Other Comments:

    A good overall presentation. This technique will certainly be of great use to other researchers. The style of presentation and the experimental techniques used are of acceptable quality.  The author must put in details concerning approval from Animal Ethics committee since work was carried out on animals. The number of animal used should also be clearly mentioned.

  • Competing interests:
    none
  • Invited by the author to review this article? :
    No
  • Have you previously published on this or a similar topic?:
    No
  • References:
    None
  • Experience and credentials in the specific area of science:

    I work with various cell culture techniques and animal models in cancer research

  • How to cite:  Abdul Majid A .An efficient approach to enrich glioma stem cells from glioma cell lines in culture [Review of the article 'An efficient approach to Enrich Glioma Stem Cells from Glioma Cell Lines in Culture ' by Kamnasaran D].WebmedCentral 2010;1(12):WMCRW00224
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